Oxidized dextran coated magnetic nanoparticles to develop magnetic cross-linked Bacillus lehensis G1 endolevanase aggregates for levan-type fructooligosaccharides synthesis.

ABSTRACT: Endolevanase from Bacillus lehensis G1 (rlevblg1) is an enzyme that hydrolyzes levan for the production of levan-type fructooligosaccharides (L-FOS). To maximize the industrial utility of enzymes, it is essential to find effective immobilization techniques that preserve their activity and stability. This study introduced the use of in silico analysis to predict the attachment site in order to perform site-directed cross-linking and develop efficient magnetic cross-linked enzyme aggregates (M-CLEAs). According to the computational analysis, dextran with aldehyde groups has attached far from the active site of rlevblg1 compared to dextran with acetyl functional groups. Oxidized dextran (Odex) was prepared and used to coat the magnetic nanoparticles (MNPs). The coated-MNPs (OdexM) exhibited s

SUBMITTER: Makki HH 

PROVIDER: S-EPMC12527180 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature