STAG2 regulates polycomb and differentiation in urothelial precursors and bladder cancer.

ABSTRACT: The STAG2 tumor suppressor gene is commonly inactivated by mutations in a wide range of common cancer types. STAG2 encodes a component of the cohesin complex, which controls sister chromatid cohesion and 3D genome organization. In bladder cancer, STAG2 mutations are most common in the earliest low-grade lesions, suggesting that mutational inactivation of STAG2 may be an initiating event. To provide insight into the mechanisms of STAG2 tumor suppression in bladder cancer, siRNA and shRNA were used to knock down STAG2 in several different human non-neoplastic bladder cancer precursor cell lines. Gene editing was used to generate cultured human cancer cell lines that differ only in the presence or absence of bladder-cancer derived STAG2 mutations. These systems were interrogated using RNA-seq

SUBMITTER: Park Y 

PROVIDER: S-EPMC12527211 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature