Combination of targeted pharmacotherapy and immunotherapy with anti-CD19 CAR NK cells in acute lymphoblastic leukemia.

ABSTRACT: Anti-CD19 CAR NK cells may provide a promising non-HLA-restricted immune cell product and have been clinically studied primarily on low-grade B-cell lymphoma patients. We used retroviral gene transfer to generate aCD19 CAR NK cells from the peripheral blood of healthy volunteers. We evaluated their efficacy in B-lineage acute lymphoblastic leukemia (BCP-ALL) using patient-derived xenograft (PDX) cells in vitro and in vivo. aCD19 CAR NK cells showed potent specific cytotoxicity against eleven BCP-ALL PDX models in vitro. When used as monotherapy in vivo, they provided a survival benefit, albeit complete remissions were not achieved. Due to the low accumulation of aCD19 CAR NK cells in the bone marrow, we used targeted pharmacotherapy based on venetoclax, dexamethasone, and dasatinib to indu

SUBMITTER: Kirchhoff H 

PROVIDER: S-EPMC12527222 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature