Label-free fluorescence lifetime imaging for real-time guidance of stereotactic biopsy: A feasibility study in brain cancer patients.

ABSTRACT:

Background

Stereotactic needle biopsy is frequently indicated for the diagnosis of malignant gliomas in initial management and at tumor recurrence. Current techniques have a sampling error of 24% and diagnostic errors of 10%-30%. Fluorescence lifetime imaging (FLIm) distinguishes among types of brain tissue according to their intrinsic fluorescence properties. Intraprocedural FLIm has been implemented for biopsy guidance.

Methods

A FLIm imaging system (fiber-optic probe; pulsed excitation: 355 nm; avalanche photodiode (APD)-based detection: 470/28 nm, 540/50 nm) linked to a stereotactic navigation platform was used to image tissue through the 10-mm side opening of a 1-mm-diameter needle during MRI-directed stereotactic biopsy of 5 suspected malignant gliomas. As the biopsy n

SUBMITTER: Alfonso-Garcia A 

PROVIDER: S-EPMC12527272 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature