Ovarian SUMO-2/3 targets and their differential response to genotoxic stress induced by 7,12-dimethylbenz(a) anthracene exposure in lean and obese female mice†.

ABSTRACT: SUMOylation is a post-translational modification critical for oocyte development and chromatin-associated processes. Environmental exposures and obesity can cause follicle depletion and exposure to 7,12-dimethylbenz(a)anthracene (DMBA) altered ovarian small ubiquitin-like modifiers (SUMO) protein abundance in lean and obese mice. Thus, the hypothesis that exposure to DMBA may alter ovarian protein SUMOylation as a mode of ovotoxicity dampened by obesity was tested. Lean and obese mice (KK.Cg-a/a and KK.Cg-Ay/J) were exposed to either corn oil or DMBA (1 mg/kg) intraperitoneally for 7d (n = 4/treatment) and ovaries were flash-frozen on the second day of diestrus. Protein was isolated followed by immunoprecipitation using a SUMO-2/3 antibody and precipitated proteins were identified via liqu

SUBMITTER: Rishi JK 

PROVIDER: S-EPMC12527294 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature