Optimizing HLA desensitization: serum dilution strategies and platform-specific MFI thresholds for antibody-mediated rejection risk in kidney transplantation.

ABSTRACT:

Introduction

Highly sensitized kidney transplant (KT) candidates are at increased risk of antibody-mediated rejection (ABMR), particularly in the context of desensitization protocols. Accurate quantification of donor-specific antibodies (DSA) using Luminex single antigen bead (SAB) assays remains essential but is limited by signal saturation and inter-platform variability.

Methods

We conducted a bicentric study involving 20 sensitized KT recipients undergoing HLA desensitization. Anti-HLA antibody profiles were analyzed using Immucor- Werfen (IM) and One Lambda-Thermofisher (OL) SAB platforms. Serum samples were tested both undiluted and at 1:10 dilution before and after desensitization.

Results

Diluted pre-desensitization sera showed stronger correlation with post-de

SUBMITTER: Bertrand D 

PROVIDER: S-EPMC12527829 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature