Transepidermal water loss increases during murine food anaphylaxis and reflects reaction severity.

ABSTRACT:

Background

An increase in transepidermal water loss (TEWL) presages food anaphylaxis in allergic humans during oral food challenges. We sought to determine whether similar TEWL changes occur in mouse food anaphylaxis models.

Methods

Using a Tewameter™ Nano, a mouse-compatible device, TEWL measurements were conducted on the ear, paw, and abdomen of BALB/c mice. Because of the highest measurement reproducibility, the ear was selected for use in the study. Baseline TEWL measurements under varied conditions were evaluated. Histamine injections were given to evaluate a non-IgE-mediated reaction. Two IgE-based models of food anaphylaxis were utilized: (1) passive systemic anaphylaxis (PSA) with dinitrophenyl (DNP)-IgE sensitization and DNP-albumin challenge, and (2) active systemi

SUBMITTER: Woerner BN 

PROVIDER: S-EPMC12528144 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature