Behavioral benefits of GSK-3β inhibition and state-dependent microtubule signatures in the <i>Fmr1</i>-KO mouse.

ABSTRACT: Glycogen-synthase-kinase-3β (GSK-3β) and microtubule dynamics are implicated in Fragile X syndrome (FXS). We examined behaviors and hippocampal α-tubulin post-translational modifications (PTMs) in Fmr1-KO male mice without and with chronic administration of the GSK-3β inhibitors SB216763 (30 mg/kg, i.p.) and AF3581 (10 mg/kg, i.p.). Fmr1-KO male mice and wild-type (WT) were evaluated in the open field, marble-burying, elevated-plus-maze (EPM), novel-object-recognition (NOR) and three-chamber sociability test (3-CST); acetylated α-tubulin (Acet/Total-Tub) and tyrosinated/detyrosinated α-tubulin (Tyr/Glu-Tub) ratios were then analyzed. Fmr1-KO male showed hyperactivity, excessive marble burying and impaired NOR; Acet/Total-Tub was elevated and Tyr/Gl

SUBMITTER: Kealy J 

PROVIDER: S-EPMC12528152 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature