Multicenter evaluation of fast multiplex PCR for detection of pathogens in lower respiratory tract infections.

ABSTRACT:

Background

This study aimed to compare the diagnostic performance between bacterial culture and a rapid multiplex PCR assay (Respiratory Pathogens Multiplex Nucleic Acid Diagnostic Kit) for detecting six bacterial (Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae, Haemophilus influenzae, Legionella pneumophila) and six viral targets (Influenza A/B, Respiratory syncytial virus, Adenoviruses, Human rhinovirus, Mycoplasma pneumoniae) in 728 bronchoalveolar lavage (BAL) specimens.

Methods

This multicentric observational study was conducted at six comprehensive large hospitals in Hunan Province, China (May to October 2023) and assessed performance of mPCR kit by comparison with conventional culture method.

Results

The mPCR kit

SUBMITTER: Wang L 

PROVIDER: S-EPMC12528164 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature