Clinical isolates of <i>Providencia rettgeri</i> and <i>Providencia Stuartii</i> evades neutrophil-mediated killing by subverting neutrophil-extracellular traps.

ABSTRACT:

Introduction

Providencia rettgeri (Pr) and Providencia stuartii (Ps) are clinically relevant opportunistic pathogens. They are resistant to several antibiotics including carbapenems. The immune response against these pathogens has never been investigated. Here, we aimed to evaluate whether neutrophils (PMN), key players against bacterial infections, were able to recognize and eliminate these bacteria.

Methods

We measured PMN functions after challenge with selected clinical isolates of Pr and Ps, and used Escherichia coli ATCC (Eco), which fully activates PMN, for comparison. Bacterial survival was evaluated after exposure of PMN to bacteria for 1 or 3 h and colony formation units (CFU) were determined.

Results

While PMN were able to partially cont

SUBMITTER: Castro JE 

PROVIDER: S-EPMC12528210 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature