Development of CRISPR/Cas9-mediated CD16b^-/- and CD32a^-/- promyelocytic cell lines to study FcγR signaling in human neutrophils.

ABSTRACT:

Introduction

Neutrophils use Fc gamma receptors (FcγRs) to recognize IgG-opsonized pathogens, triggering antimicrobial functions including phagocytosis, ROS production, and cytokine release. CD16b, the most abundant FcγR on neutrophils, plays a key role in initiating these responses, while CD32a is another abundant FcγR on neutrophils that contributes to modulating immune functions. CD16b lacks an intracellular domain and its signaling mechanisms remain unclear. The prevalence of the CD16b-deficient phenotype on donor neutrophils is estimated at <1% of the global population, which complicates its study. To address this, we employed CRISPR/Cas9 to generate HL-60-derived neutrophil-like cells deficient for CD16b or CD32a, that facilitate investigation of their respective roles in neu

SUBMITTER: Cruz-Cardenas JA 

PROVIDER: S-EPMC12528219 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature