SOX4 mediates cancer-associated fibroblasts related radioresistance in hepatocellular carcinoma.

ABSTRACT:

Purpose

Cancer-associated fibroblasts (CAFs), a crucial component of tumor microenvironment, play a critical role in tumorigenesis, progression, and conferring resistance to radiotherapy and chemotherapy. This study aimed to investigate the association between CAFs, CAF- related genes, and radioresistance in hepatocellular carcinoma (HCC).

Methods

CAFs were isolated from HCC tissues and subsequently utilized for co-culturing with HCC cells using CAFs-conditioned medium. An orthotopic HCC mouse model was established by co-implanting CAFs and HCC cells. Through integrative analysis of three RNA-sequencing datasets (TCGA-LIHC tumor vs. normal tissues, Huh7 radioresistant vs. parent cells, and CAF vs. control group), CAF-associated prognostic genes were identified using comprehe

SUBMITTER: Jiang K 

PROVIDER: S-EPMC12528248 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature