Integrating multi-modal transcriptomics identifies cellular subtypes with distinct roles in PDAC progression.

ABSTRACT:

Purpose

Pancreatic ductal adenocarcinoma (PDAC) remains one of the deadliest malignancies, largely due to its highly immunosuppressive and fibrotic tumor microenvironment (TME). However, the spatial and functional organization of its cellular components remains poorly understood.

Methods

We present an integrated transcriptomic atlas of the PDAC TME by combining single-cell RNA sequencing (n = 88; 187,520 cells), Visium spatial transcriptomics (n = 20; 67,933 spots), bulk RNA sequencing (n = 1,383), and high-resolution Xenium spatial transcriptomics (n = 2; 307,679 cells). Key findings were validated using scRNA-seq, bulk datasets, multiplex immunohistochemistry, and spatial imaging.

Results

POSTN⁺ fibroblasts and SPP1⁺ macrophages consistently co-infiltrated across 12

SUBMITTER: Wu J 

PROVIDER: S-EPMC12528271 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature