COPB2 facilitates EDEM3-mediated mannose trimming to sustain ER homeostasis in ovarian cancer.

ABSTRACT:

Background

Ovarian cancer (OC) is a lethal gynecologic malignancy with limited therapeutic success due to late diagnosis and therapy resistance. Endoplasmic reticulum (ER) stress and ER-associated degradation (ERAD) are key to tumor adaptation, yet the mechanisms sustaining ER homeostasis in OC remain poorly defined.

Methods

We combined multi-omics analyses, tissue microarrays, and in vitro and in vivo models. Functional assays involved COPB2 knockdown or overexpression in OC cells, xenografts in nude mice, and mechanistic studies including protein interaction and glycoproteomic analyses.

Results

COPB2 was significantly upregulated in OC and associated with poor prognosis. It promoted cell proliferation and survival by alleviating ER stress and suppressing apoptosis.

SUBMITTER: Li DX 

PROVIDER: S-EPMC12528345 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature