The oncogene protein kinase PIM1 regulates mammalian erythroblast enucleation.

ABSTRACT: Erythroblast enucleation is a unique process during mammalian erythropoiesis, yet its regulatory mechanisms remain largely elusive. Here, we demonstrate the specific regulatory role of the oncogene PIM1, the most highly expressed protein kinase in orthochromatic erythroblasts, in enucleation. Unlike its well-established roles in cancer cell proliferation and survival, knockdown of PIM1 in human erythroid cells does not affect cell growth or apoptosis, but specifically inhibits erythroblast enucleation without altering differentiation. To elucidate the functional conservation of PIM1 in mammalian erythropoiesis, we generate Pim1^fl/flEpoR^Cre mice in which Pim1 is deleted in erythroid cells. Consistent with human erythropoiesis, deletion of Pim1 in mice has no detectable

SUBMITTER: Zhang H 

PROVIDER: S-EPMC12528402 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature