Fast detection of synergy and antagonism in antifungal combinations used against Candida albicans clinical isolates.

ABSTRACT: The rise in antifungal resistance has limited treatment options for serious fungal infections, emphasizing the need for effective combination therapies. However, low-cost and rapid systems to evaluate synergy and antagonism in antifungal combinations are lacking. Here, we introduce a novel in vitro testing method for assessing antifungal interactions in C. albicans, enabling the simultaneous testing of three antifungal agents in a single agar plate with overnight results. This method, validated against the checkerboard assay, provides consistent fractional inhibitory concentration (FICi) measurements with reduced variability and workload. We applied this method in a comprehensive screen of 92 clinical C. albicans isolates for three antifungals-amphotericin B, fluconazole, and anidulafungin

SUBMITTER: Lundvik H 

PROVIDER: S-EPMC12528425 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature