Protective effect of tanshinone IIA against Listeria monocytogenes infection in vitro and in vivo.

ABSTRACT: Listeriolysin O (LLO) is a vital virulence factor for the intracellular replication and pathogenesis of Listeria monocytogenes. The search for an effective LLO activity inhibitor as an alternative approach for the treatment of L. monocytogenes infection has attracted great attention. Tanshinone IIA (Tan IIA) is a bioactive compound in the traditional Chinese medicine "Danshen". In this study, the protective effect of Tan IIA against L. monocytogenes infection was investigated using hemolysis test, LDH release method, oligomerization assay and molecular dynamics simulation in combination with in vivo mouse experiment. Tan IIA significantly inhibited the pore-forming activity of LLO by forming a stable LLO - Tan IIA complex. Meanwhile, Tan IIA concentration-dependently decreased the cytotoxi

SUBMITTER: Wang H 

PROVIDER: S-EPMC12528476 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature