GNAS/PKA signaling promotes aberrant osteochondral differentiation of Gli1⁺ tendon sheath progenitors.

ABSTRACT: Tendon injury promotes aberrant osteochondral differentiation of tendon stem cells (TSCs) and results in disability. However, the cellular subsets within the osteochondral lineage involved in this process and associated mechanisms remain unclear. Here, we found that, following Achilles tenotomy, murine Gli1⁺ tendon sheath cells expanded rapidly, transitioning into tenogenic and osteochondrogenic cells. Lineage tracing, together with single-cell RNA sequencing, revealed that osteochondrogenic Gli1⁺ tendon sheath cells originate from Scx⁺ tendon stem/progenitor cells, preferentially differentiate into osteochondral lineage tendon progenitors at 7 dpi, subsequently undergoing aberrant chondrogenesis and osteogenesis at 21dpi and 63dpi, respectively. In additio

SUBMITTER: Chen L 

PROVIDER: S-EPMC12528478 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature