Differential Chromatin Accessibility, Gene Expression, and mRNA Splicing Between Developing Cochlear Inner and Outer Hair Cells.

ABSTRACT:

Purpose

The mammalian cochlea has two types of low abundance and highly specialized inner (IHC) and outer (OHC) mechanosensory hair cells. Their malfunction or death is a common cause of congenital and acquired deafness. IHCs and OHCs exhibit different transcriptomes during development. We wondered how differences in gene expression are regulated at the chromatin level in developing IHCs and OHCs, and whether there were also differences in mRNA splicing between IHCs and OHCs.

Methods

We separately collected developing mouse IHCs and OHCs to identify their mRNAs and chromatin states. We examined their transcriptomes by bulk (full coverage) RNA-seq from six biological replicates each to reveal differences in gene expression and in alternative mRNA splicing. We also examined th

SUBMITTER: Foo CZ 

PROVIDER: S-EPMC12528612 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature