Suppressing CDCA8/CDK1 improves oral squamous cell carcinoma by modulating proliferation, apoptosis, cell cycle and migration.

ABSTRACT: We aimed to clarify the role of CDCA8/CDK1 in the progression of oral squamous cell carcinoma (OSCC) and the potential mechanisms. To explore the CDCA8 expression in OSCC, clinical samples were collected. In vitro, CAL-27 cells were transfected with short hairpin RNA (shRNA) to silence CDCA8, and then proliferation, apoptosis, cell cycle and migration were evaluated. KEGG analysis was used to explore the causal association of CDCA8 and CDK1 during the development of OSCC. CDK1 inhibitor RO3306 and overexpression of CDK1 were used to clarify the regulatory role of CDK1 in OSCC. In vivo, mice were injected with shCDCA8 to deplete CDCA8 based on establishing a model of OSCC. We found that CDCA8 was highly expressed in OSCC tissues and cell lines. In vitro experiments, suppressing CDCA8 in CAL

SUBMITTER: Zhu J 

PROVIDER: S-EPMC12528644 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature