Spatial biology using single-cell mass spectrometry imaging and integrated microscopy.

ABSTRACT: Spatial biology pursues the analysis of cells in their native microenvironment, often with regard to morphology and gene- or protein expression. The spatial analysis of lipid and metabolic profiles by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can add another layer of molecular information. A seamless integration of MSI data with other (multimodal) methods at a single-cell level is often hampered by insufficient co-registration and resolution. Here, we introduce a MALDI-MSI based method that integrates in-source bright-field and fluorescence microscopy, allowing for a coupled (sub-)cellular investigation of the same sample in both modalities. Presented examples from cell culture and tissue analysis include the visualization of intracellular lipid dist

SUBMITTER: Potthoff A 

PROVIDER: S-EPMC12528677 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature