Assessment of the safety of hypoxia-primed mesenchymal stem cells derived from umbilical cord and adipose tissues in animals.

ABSTRACT: Mesenchymal stem cells (MSCs) are widely used for disease treatment, typically cultured in ambient oxygen (21%) exceeding physiological levels (2-5%). While hypoxic MSCs show in vitro advantages, their in vivo effects remain less understood. This study evaluated the safety of xeno-free, serum-free cultured MSCs from adipose tissue (AD-MSCs) and umbilical cords (UC-MSCs) primed with 5% oxygen in healthy rabbits, Swiss mice, and rats. Assessments included vascular and muscle stimulation, systemic hypersensitivity, acute toxicity, and subchronic toxicity. Our data indicated that the injection of UC-MSCs induced vascular stimulation but not muscular stimulation, whereas the injection of AD-MSCs caused neither vascular nor muscular stimulation. Additionally, neither AD-MSCs nor UC-MSCs affected

SUBMITTER: Dinh NT 

PROVIDER: S-EPMC12528746 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature