A diverse semisynthetic humanized scFv phage display library for anti-CXCL16 antibodies.

ABSTRACT: Phage display libraries of human single-chain variable fragments (scFvs) serve as a valuable resource for generating fully human antibodies for scientific and clinical applications. In this study, we designed and constructed a highly diverse semisynthetic humanized scFv phage display library using an optimized Kunkel mutagenesis approach. Our optimizations eliminated residual template, enhancing mutagenesis efficiency and expanding library diversity with a reservoir capacity exceeding 10¹⁰. For this semisynthetic library, the complementarity-determining region 3 was structurally designed to mimic the natural human antibody repertoires, encompassing comprehensive sequence variability and length distributions. To functionally characterize this antibody library, we performed target

SUBMITTER: Li Z 

PROVIDER: S-EPMC12528905 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature