Safety evaluation of the food enzyme mannan endo-1,4-β-mannosidase from the non-genetically modified <i>Aspergillus niger</i> strain ACH 12-525.

ABSTRACT: The food enzyme mannan endo-1,4-β-mannosidase (1,4-β-D-mannan mannanohydrolase; EC 3.2.1.78) is produced with the non-genetically modified Aspergillus niger strain ACH 12-525 by Shin Nihon Chemical Co., Ltd. The food enzyme was considered free from viable cells of the production organism. The food enzyme is intended to be used in three food manufacturing processes. Dietary exposure to the food enzyme-total organic solids (TOS) was estimated to be up to 0.579 mg TOS/kg body weight (bw) per day in European populations. Genotoxicity tests did not indicate a safety concern. The systemic toxicity was assessed by means of a repeated dose 90-day oral toxicity study in rats. The Panel identified a no observed adverse effect level of 1331 mg TOS/kg bw per day, the highest dose tested, which

SUBMITTER: EFSA Panel on Food Enzymes (FEZ) 

PROVIDER: S-EPMC12528993 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature