Large-scale quantification of stomatal patterning in barley leaves overexpressing epidermal patterning factor 1 reveals differential stomatal density between the adaxial and abaxial surfaces and spatial heterogeneity that impact stomatal function.

ABSTRACT: Stomatal density varies spatially over the leaf surface and between abaxial and adaxial leaf surfaces, with distribution greatly influencing plant photosynthesis and water use. However, methodological limitations have prevented quantification of spatial heterogeneity and its consequences for gaseous exchange in monocot crops. Here we introduce a simple and rapid method to image and quantify stomatal patterning over large (18 cm²) leaf areas in situ. We used this approach to assess spatial variation across the adaxial and abaxial surfaces in barley (Hordeum vulgare L.) wild-type (WT) plants and mutants overexpressing the epidermal patterning factor 1 (EPF1). Analysing over a million stomata revealed significantly greater stomatal densities on the adaxial surface and towards the l

SUBMITTER: Fan M 

PROVIDER: S-EPMC12529052 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature