Online Monitoring of Chip-Based Microscale Perfusion Fermentations.

ABSTRACT: As part of established biomanufacturing development, screening and early phase bioprocess development occurs at bench scale (microplates and shake flasks) whereby conventional offline sampling can only provide limited feedback on fermentation bioprocess parameters including strain productivity. To address these limitations, a new sensitive and selective online analytical platform consisting entirely of commercially available components with a small footprint (valves, 2DLC hardware, LC separation, and online tandem mass spectrometry) was developed for online monitoring of chip-based microbioreactors. Fermentations of microbial cell factories (Saccharomyces cerevisiae) were cultivated in 20 μL bioreactors, requiring perfusion of cell culture media at low μL/min rates delivered by syri

SUBMITTER: Cramer SM 

PROVIDER: S-EPMC12529116 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature