Developing a Human Immune-Endothelial Coculture Model for Inflammatory Vasculitis and Drug Screening.

ABSTRACT: Vasculitis is an inflammatory condition characterized by immune cell activation and vascular endothelial dysfunction leading to severe clinical complications, including cytokine release syndrome. Despite existing treatment strategies such as corticosteroids and NSAIDs, severe cases remain difficult to manage, necessitating improved in vitro models for mechanistic studies and drug evaluation. This study established a direct coculture model of human umbilical vein endothelial cells (HUVECs) and peripheral blood mononuclear cells to investigate immune-endothelial cell interactions under lipopolysaccharide (LPS)-induced inflammatory conditions. Upon LPS stimulation, the cocultured HUVECs exhibited increased expression of inflammatory cytokines (IL-6 and IL-8) and adhesion molecules (VCAM-1 and

SUBMITTER: Kyun ML 

PROVIDER: S-EPMC12529162 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature