Detection and Quantitation of Small Proteins Using Mass Spectrometry.

ABSTRACT: Small proteins or microproteins, despite long being ignored, have important roles in the regulation of larger protein complexes, metabolic pathways, and gene expression. However, these proteins remain under-represented in proteomics studies because of low detection efficiency in traditional mass spectrometry (MS) workflows. Their inherent characteristics often lead to depletion during sample preparation and a low detection efficiency in LC-MS. To improve detection and quantitation, we took advantage of the large set of documented small proteins in Escherichia coli and systematically compared and optimized different sample preparation and LC-MS approaches. We evaluated different top-down and bottom-up approaches, including data-dependent acquisition, data-independent acquisition, and parall

SUBMITTER: Franco PHC 

PROVIDER: S-EPMC12529367 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature