Construction and Characterization of Novel Shuttle Expression Vectors for Actinobacillus succinogenes.

ABSTRACT: Actinobacillus succinogenes is a ruminal microorganism of biotechnological importance due to its capacity to produce succinic acid at high yields. Despite the scientific interest in this organism, molecular vehicles for the transfer and expression of genetic material are limited compared to the existing demand. To facilitate gene cloning and expression in A. succinogenes, we report the generation and characterization of two novel shuttle expression vectors containing the chloramphenicol acetyltransferase gene (catA) as a selection marker and replication origins from A. succinogenes, other members of the Pasteurellaceae family, and Escherichia coli. Vector pAVP_trc includes the following features from the E. coli expression vector pTrc99A2: lacI^q gene, an IPTG-inducible

SUBMITTER: Vallejo-Giraldo A 

PROVIDER: S-EPMC12529458 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature