PLANTA Protocol for the Direct Detection and Identification of Bioactive Compounds in Complex Mixtures via Combined NMR-HPTLC-Based Heterocovariance.

ABSTRACT: The assignment of bioactivity to compounds within complex natural product (NPs) mixtures remains a significant challenge in NPs research. The present research introduces a comprehensive protocol, named "PLANTA (PhytochemicaL Analysis for NaTural bioActives)" protocol, for the detection and identification of bioactive compounds in complex natural extracts prior to isolation combining the NMR-HeteroCovariance Approach (NMR-HetCA), high-performance thin-layer chromatography (HPTLC), and chemometric techniques. This study emphasizes two novel components: STOCSY-guided targeted spectral depletion, adapted to resolve overlapping NMR signals in complex matrices, improve minor component detection, and facilitate identification through

SUBMITTER: Amountzias V 

PROVIDER: S-EPMC12529470 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature