A Flexible End-to-End Automated Sample Preparation Workflow Enables Standardized and Scalable Bottom-up Proteomics.

ABSTRACT: Bottom-up proteomics holds significant promise for clinical applications due to its high sensitivity and precision but is limited by labor-intensive, low-throughput sample preparation methods. Advanced automation is essential to enhance throughput, reproducibility, and accuracy and to allow standardization to make bottom-up proteomics amenable to large-scale studies. We developed a fully integrated, automated sample preparation platform that covers the entire process from biological sample input to mass-spectrometry-ready peptide output and can be applied to a multitude of biological samples. With this end-to-end solution, we achieved high intra- and interplate reproducibility, as well as longitudinal consistency, resulting in precise and reproducible workflows. We showed that our automate

SUBMITTER: Schar S 

PROVIDER: S-EPMC12529477 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature