Divergent accumulation patterns of SNVs and INDELs reveal negative selection in noncancerous cells.

ABSTRACT: Somatic mutations accumulate with age in human tissues. Clonal amplification of some mutations causes cancers and other diseases. However, it is unclear if random mutation accumulation affects cellular function without clonal amplification. We tested this in cell culture, avoiding the limitation that mutation accumulation in vivo leads to cancer. We performed single-cell whole-genome sequencing of fibroblasts from DNA-mismatch-repair-deficient Msh2 ^-/- mice and controls after long-term passaging. While maintaining the same growth rates, in the Msh2 ^-/- fibroblasts, single-nucleotide variants increased up until >50,000 per cell, with small insertions and deletions plateauing at ∼16,000 per cell. We provide evidence for genome-wide negative selectio

SUBMITTER: Zhang L 

PROVIDER: S-EPMC12529607 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature