Demonstration and structural basis of a therapeutic DNA aptamer for SARS-CoV-2 spike protein detection.

ABSTRACT: At the onset of the COVID-19 pandemic, the absence of rapid and precise diagnostic tools hindered early detection and response. To address this challenge, we developed a renewable electrochemical impedance biosensor (aptasensor) using a therapeutic DNA aptamer immobilized on a nanostructured gold nanoparticle/carbon nanotube (AuNP/CNT) electrode to detect the SARS-CoV-2 spike (S) protein receptor-binding domain (RBD). The aptasensor achieved a limit of detection of 0.19 pg mL^-1 and a dynamic range from 1 to 10⁵ pg mL^-1. Following regeneration with a 60-s pH 2.0 rinse, the sensor retained over 90% of its original signal across five cycles and remained stable after two weeks of ambient storage. Dual-mode readouts, utilizing impedance spectroscopy and surface

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12529732 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature