Mitomycin C Induces Autophagy in Human Tracheal Fibroblasts and Suppresses Their Growth.

ABSTRACT:

Objective

Mitomycin C (MMC) is frequently used to prevent postoperative fibrosis in tracheal stenosis, yet its precise cellular mechanisms remain inadequately understood. This study aimed to elucidate the cytotoxic and autophagic effects of MMC on normal human tracheal fibroblasts (hTF) and human bronchial/tracheal epithelial cells (hTEC) to better understand its potential role in fibrosis regulation.

Methods

hTF and hTEC were exposed to MMC at concentrations of 0.01, 0.1, and 1 μg/mL for 24, 48, and 72 h. Cell proliferation, autophagy induction, and the expression of autophagy-related proteins were assessed using viability assays and Western blot analysis. Additionally, the effects of MMC on cell migration and fibroblast-to-myofibroblast transition were investigated.

Res

SUBMITTER: Kim JM 

PROVIDER: S-EPMC12529875 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature