Metformin improves immunosuppressant induced hyperglycemia and exocrine apoptosis in rats.

ABSTRACT:

Background

Immunosuppressants are an important cause of posttransplantation diabetes mellitus. We have shown that tacrolimus and sirolimus induce hyperglycemia and hyperinsulinemia in normal rats. We hypothesized that metformin, given concurrently with tacrolimus and/or sirolimus, prevents disturbances in glucose and insulin metabolism.

Methods

Eight groups (n=6) of normal Sprague-Dawley rats were studied: four groups received tacrolimus, sirolimus, tacrolimus/sirolimus, or control for 14 days, and four more groups received similar treatments along with metformin. Daily glucoses were measured. All rats were administered an oral glucose challenge before sacrifice. Pancreata were analyzed by terminal deoxynucleotide tranferase-mediated dUTP nick-end labeling staining and immun

SUBMITTER: Shivaswamy V 

PROVIDER: S-EPMC12529965 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature