Rtpcr: a package for statistical analysis and graphical presentation of qPCR data in R.

ABSTRACT:

Background

Quantitative real-time polymerase chain reaction (qRT-PCR or qPCR) is widely used in molecular biology research. Various analysis methods are employed to interpret qPCR data and measure mRNA levels of target genes under different experimental conditions.

Results

The rtpcr package was developed for amplification efficiency calculation, statistical analysis, and graphical presentation of qPCR data in R. It uses a general calculation methodology that accommodates up to two reference genes and amplification efficiency values, including the Pfaffl method. Depending on the experimental design, rtpcr functions apply a t-test (for experiments with a two-level factor), analysis of variance (ANOVA), or analysis of covariance (ANCOVA) (for experiments with more than t

SUBMITTER: Mirzaghaderi G 

PROVIDER: S-EPMC12530193 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature