Navigating The CeRNA Axis by Bioinformatics and Experimental Validation: Identification of <i>ADIRF-AS1</i>, miR-191-5p, and EGR1 as Key Players in Endometrial Carcinoma Progression.

ABSTRACT:

Background

Endometrial carcinoma (EC) is a significant gynecologic malignancy. Investigating competing endogenous RNA (ceRNA) networks, including long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs), offers insights into EC's molecular intricacies and may improve therapeutic and diagnostic strategies. This study focuses on the ceRNA axis, particularly the interactions of lncRNA-miRNA-mRNA-Gene, using TCGA-UCEC database analysis and experimental validation.

Materials and methods

In this case-control study, differentially expressed lncRNAs (DElncRNAs), microRNAs (DEMIs), and genes (DEGs) were identified, highlighting ADIRF-AS1 as a potential target. Validated interactions between ADIRF-AS1, miR-191-5p, and EGR1 were established, with significant ADIRF

SUBMITTER: Rezaei A 

PROVIDER: S-EPMC12530210 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature