Comparison the impact of IFN-β alone or in combination with vitamin D on critical pathways involved in AML progression.

ABSTRACT:

Background

Acute myeloid leukemia (AML) is a malignant disorder characterized by the accumulation of immature myeloid cells which can be developed and exacerbated through inflammation. Interferon-β (IFN-β) and vitamin D (Vit D) are known for their immunomodulatory and anti-proliferative effects. Key molecules like IL-1β, Gal-9, β-catenin, and NF-κB play important roles in AML progression. This study examines the effects of IFN-β alone and in combination with Vit D on U937 cell proliferation and the regulation of these key molecules.

Methods

Cell counting kit-8 (CCK-8) was applied to assess the effect of IFN-β and Vit D on U937 cells proliferation. Real-time PCR was carried out to evaluate the impact of IFN-β and Vit D on IL-1β, IL-10, Gal-9, β-catenin, and NF-κB genes. ELISA

SUBMITTER: Nasri F 

PROVIDER: S-EPMC12530539 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature