Optimization and Validation of a Folate Ligand-Targeted qPCR Method for Detection of Folate Receptor-Positive Circulating Tumor Cells in Gastric Cancer.

ABSTRACT:

Background

Gastric cancer represents a significant global health concern with poor prognosis and limited early detection methods. Circulating tumor cells (CTCs) that express folate receptors (FRs) have emerged as promising biomarkers for cancer monitoring, as folate receptor-alpha is overexpressed in cancer tissues while remaining absent in normal cells.

Objective

This study aimed to develop and optimize a Folate-Ligand Targeted quantitative PCR (Folate-LT qPCR) method for the selective detection and quantification of FR+ CTCs in gastric cancer patients.

Methods

The methodology involved designing a folate-oligonucleotide conjugate with a 5' folate moiety, triethylene glycol spacer, and phosphorothioate bond for stability. A specific stem-loop primer was developed for

SUBMITTER: Suwa A 

PROVIDER: S-EPMC12530657 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature