Nonsurgical Retreatment of a 16 × 9 mm Osteolytic Lesion With Buccal Bone Regeneration Using a Bioceramic Sealer: A Clinical and NGS-Based Microbiological Case Report With 30-Month Follow-Up.

ABSTRACT: This case report described the complete resolution of a large osteolytic lesion following a nonsurgical endodontic retreatment using a premixed bioceramic sealer. A 30-year-old healthy patient presented a large radiographic osteolytic lesion of 16 × 9 mm in correspondence with Teeth #42, #41, and #31. Clinical examinations revealed positive percussion tests and increased mobility of all incisors. The microbiological profile of the root canals was assessed through paper point sample collection, DNA extraction, and next-generation sequencing, revealing markedly different proportions of bacteria genera in the affected teeth. A secondary endodontic treatment was then performed, combining manual, rotary, and reciprocating protocols. A premixed bioceramic-based sealer (CeraSeal) was used for obt

SUBMITTER: Spinelli A 

PROVIDER: S-EPMC12530936 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature