Investigating the mechanisms of indocyanine green tumour uptake in sarcoma cell lines and ex vivo human tissue.

ABSTRACT: Indocyanine green (ICG) is a well-established near-infrared dye which has been used clinically for several decades. Recently, it has been utilised for fluorescence-guided surgery in a range of solid cancer types, including sarcoma, with the aim of reducing the positive margin rate. The increased uptake and retention of ICG within tumours, compared with normal tissue, gives surgeons a visual reference to aid resection when viewed through a near-infrared camera. However, the mechanisms of this process are poorly understood. We performed in vitro ICG cellular uptake studies across a panel of sarcoma cell lines exhibiting varying proliferation rates and phenotypes. The effects of ICG concentration, incubation time, inhibition of clathrin-mediated endocytosis, and cell line proliferation rate o

SUBMITTER: Chan CD 

PROVIDER: S-EPMC12531117 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature