Decoding monocyte heterogeneity in sepsis: a single-cell apoptotic signature for immune stratification and guiding precision therapy.

ABSTRACT:

Background

The effectiveness of immunomodulatory therapies in sepsis is often hampered by profound and patient-specific immune heterogeneity. Classical monocytes play a central role in the progression toward sepsis-induced immunoparalysis, with their apoptotic rate serving as a sensitive marker of immune dysfunction. Traditional bulk transcriptomic approaches fail to resolve this complexity. Here, we harness single-cell RNA sequencing to delineate the apoptotic landscape of classical monocytes and identify robust molecular biomarkers for immunological stratification and targeted intervention.

Methods

We integrated single-cell and bulk transcriptomic data from four independent cohorts. A machine learning pipeline incorporating SVM, RF, XGB, and GLM algorithms was used to iden

SUBMITTER: Duan W 

PROVIDER: S-EPMC12531134 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature