Functional characterization of vitamin B₁₂ from an extremophile-<i>Pseudomonas alcaliphila</i> and assessment of its microbial chassis potential.

ABSTRACT:

Introduction

Vitamin B₁₂ (B₁₂) is an essential cofactor for key metabolic processes in most living organisms, yet only certain bacteria can synthesize it de novo. Common forms of B₁₂ include adenosylcobalamin (AdoCbl), methylcobalamin (MeCbl) and cyanocobalamin (CNCbl). This study presents the B₁₂ production capability of an extremophile-Ectopseudomonas alcaliphila MSJ19, and a multilevel evaluation of bioactivity of various B₁₂ forms.

Methods

B₁₂ extracted from Ectopseudomonas alcaliphila MSJ19 was initially analyzed by bioassay and LC-MS to confirm the presence of natural B₁₂ forms, followed by in vitro enzyme activity assays with glycerol dehydratase (GD) and diol dehy

SUBMITTER: Venkatesan SN 

PROVIDER: S-EPMC12531142 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature