Differential effects of immobilized CCL21 and ICAM1 on TILs with distinct expansion properties.

ABSTRACT: Adoptive T cell therapy (ACT), particularly tumor-infiltrating lymphocyte (TIL)-based therapy holds great promise for cancer treatment, yet it still faces major challenges such as patient-to-patient variability in expansion rates and cytotoxic potency. Recent studies suggest that a "synthetic immune niche" (SIN), composed of immobilized CCL21 and ICAM-1, can enhance both the expansion and cytotoxicity of murine and patient-derived T cells. To explore the mechanisms underlying the variability of expansion and cytotoxic potency, we conducted morphological and molecular phenotyping of TIL specimens from different donors immediately following the pre-Rapid Expansion Protocol (pre-REP) stage, enabling us to predict their expansion potential. We further developed novel SIN-based strategies that

SUBMITTER: Yado S 

PROVIDER: S-EPMC12531157 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature