On-tissue derivatization for mass spectrometry imaging reveals the distribution of short chain fatty acids in murine digestive tract.

ABSTRACT: Short-chain fatty acids (SCFAs), which are produced by microorganisms in the digestive tract of animals, play an important role in maintaining homeostasis in the host, including immune function. Different types of SCFAs are produced by different intestinal bacterial communities. However, visualizing their spatial distribution within tissue sections has been difficult. This is primarily due to the volatility of SCFAs, which makes detection challenging, even with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) using an atmospheric pressure ion source. To address this issue, we minimized the volatility of SCFAs in fresh tissue sections. Then, we used N,N,N-trimethyl-2-(piperazin-1-yl)ethan-1-amine iodide (TMPA) and 1-((dimethylamino)(dimethylimino)methyl)-1

SUBMITTER: Nakagawa K 

PROVIDER: S-EPMC12531170 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature