Granulocyte colony-stimulating factor induced T-cell hyporesponsiveness via modulation of CD177⁺S100A^hi neutrophils in unexplained recurrent pregnancy loss.

ABSTRACT:

Background

Numerous studies have demonstrated the promising efficacy of granulocyte colony-stimulating factor (G-CSF) in the treatment of couples with unexplained recurrent pregnancy loss (URPL) during early pregnancy. While neutrophils are recognised as the main effectors mediating immunoregulation, their G-CSF-mobilised phenotype and mechanisms regulating maternal-fetal immunity remain unclear.

Methods

Single-cell RNA sequencing (scRNA-seq) and single-cell T-cell receptor sequencing (scTCR-seq) were conducted to uncover the immune reconstitution dynamics of peripheral blood under G-CSF stimulation. Integrative analysis of transcriptomic-proteomic profiles with functional validation revealed a unique immunomodulatory neutrophil population. Further, we used spatial transcrip

SUBMITTER: Li PF 

PROVIDER: S-EPMC12531352 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature