Saliva Versus All-Site Microbiome and Proteome Mapping in Periodontitis.

ABSTRACT:

Aim

Saliva is a diagnostic surrogate for microbial and host biomarkers in periodontitis, but whether it reflects subgingival plaque, gingival crevicular fluid (GCF) or serum remains debated. This study profiled GCF and subgingival plaque from all sites of periodontitis patients, comparing them to saliva and serum.

Materials and methods

Saliva, serum, subgingival plaque and GCF were obtained from three patients with stage III, grade C periodontitis, having 23, 25 and 27 teeth, respectively, with six sites sampled per tooth. All plaque and GCF samples were pooled per patient. Shotgun sequencing and mass spectrometry proteomics were used for microbiome and proteome analysis, respectively.

Results

Totally 277 microbial taxa were collectively identified in saliva and plaqu

SUBMITTER: Bao K 

PROVIDER: S-EPMC12531364 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature