Elevated Release of Presynaptic Glutamate: The Potential Pathogenesis of Anti-NMDAR Encephalitis-Associated Seizures.

ABSTRACT:

Background

The pathogenesis of anti-N-methyl-D-aspartate receptor encephalitis (anti-NMDAR encephalitis)-associated seizures remains elusive.

Methods

Mice were injected with GluN1_359-378 peptide to construct a model of anti-NMDAR encephalitis. Next, the expression of NMDAR antibodies (NMDAR-Ab) was detected in serum samples. The electroencephalograms (EEGs) of mice were recorded. Afterward, neuronal action potentials (APs) and miniature excitatory postsynaptic currents (mEPSCs) were examined following exposure to serum derived from model mice. The expression levels of subunits of the NMDA receptor (GluN1, GluN2B) and glutamate vesicular transporter 1 (Vglut1) were quantified via Western blot analysis. Additionally, mice were injected with pentylenetetrazol (PTZ) t

SUBMITTER: Huang H 

PROVIDER: S-EPMC12531711 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature