Genome-Reduced <i>Pseudomonas putida</i> Outcompetes the Wild-Type Strain Upon Oxygen Depletion.

ABSTRACT: The broad adoption of the obligate aerobe Pseudomonas putida in industrial-scale production requires a good understanding of the effect of changing oxygen availability due to the dissolved oxygen (DO) gradients apparent at such a scale. To that end, both wild-type P. putida KT2440 and a genome-reduced derivative (strain SEM10) were subjected to different oxygen partial pressures (pO₂) in the aeration gas to evaluate the effect of low oxygen availability on growth characteristics in batch mode. Strain SEM10 consistently achieved a 12.7% higher biomass yield on glucose than the wild-type strain during non-DO limited growth, suggesting that genome reduction had no adverse effects on the overall growth properties. Furthermore, when exposed to oxygen depletion in cultiv

SUBMITTER: Jensen JW 

PROVIDER: S-EPMC12531896 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature