Transcriptomic functional characterization of recombinant adeno-associated virus producing cell line adapted to suspension-growth.

ABSTRACT: Recombinant adeno-associated virus (rAAV) is a widely used delivery vehicle in gene therapy. A scalable production technology is essential for its wide clinical applications. We have taken a synthetic biology approach to generate HEK293-based cell lines which harbor integrated genetic elements encoding essential AAV and adenoviral helper components and can be induced to produce rAAV. Through cycles of cell line enhancement, a high rAAV productivity could be achieved. The cell lines, like their parental HEK293, grew adherently. For scalable production, cell cultivation in suspension is highly desirable. A producer cell line GX6B was adapted to suspension growth in serum-free medium (named GX6Bs). However, it had substantially reduced virus titer. Returning GX6Bs cells to adherent culture co

SUBMITTER: Kuo HJ 

PROVIDER: S-EPMC12531931 | biostudies-literature | 2025 Sep-Oct

REPOSITORIES: biostudies-literature